Basic Introduction:

Automated platform for the determination of Physicochemical Properties

Elucidate drug potential

Globally renowned as the gold standard technique for determination of pKa, logP/D and solubility of ionisable compounds.

Rich insights to discovery scientists allowing them to make accurate and assured decisions about their drug candidates.

Determine pKa values using two techniques: spectrometric (for UV-active compounds) and potentiometric (for non-UV active compounds). With high throughput automated screening capabilities, SiriusT3 can complete up to 80 assays a day.

Determine the intrinsic solubility of the different forms (crystalline and amorphous) for polymorphs compounds using the Pion’s CheqSol technique.

Product Advantages:

Requiring only sub-milligram sample quantities in most cases, the SiriusT3 can complete the analysis in less than 6 minutes per titration using the high throughput pKa screening**.**

Determine pKa values using two techniques: spectrometric (for UV-active compounds) and potentiometric (for non-UV active compounds).With high throughput automated screening capabilities, SiriusT3 can complete up to 80 assays a day.‍

Measure log P/D as a function of pH in less than 2 hours using the potentiometric technique in place of traditional shake-flask methods. Determine kinetic and intrinsic solubility using Pion’s patented CheqSol technique within two hours.

Use the CheqSol technique to provide intrinsic solubility as a function of pH and generate detailed information about the extent and duration of the supersaturated states.

Product Key Features:

DTAu –Fully automated with pKa high throughput screening SiriusT3 system with both spectroscopic and potentiometric assay capabilities.

DTu – A single vial system for one assay at a time with both spectroscopic and potentiometric assay capabilities.

DTA – A fully automated system with only potentiometric assay capabilities.

DT - A single vial system for one assay at a time with only potentiometric assay capabilities.

Assay Expert – Optional integrated prediction module

Application：

Measurement of logP and logD.

Measurement of extreme pKas.

Measurement of logP for samples poorly soluble in water and octanol.

Solubility and supersaturation.

Determination of solubility - CheqSol explanation.

Detailed Specifications

Specification

pH Electrode: Ag/AgCl, double junction reference
pH Range: 1.8-12.2, calibrated via Sirius Four-Plus™
Electrode Storage: Stored in Home position; calibrated with pH 7 buffer
Stirring: Overhead, variable speed, computer-controlled
Temperature: Measured at each data point by thermocouple, Peltier controlled, 12℃-70℃
Turbidity Detection: Light transmission detection - percentage of light transmission (0.1%)
Reagents: Water, acid, base, co-solvent, dispensing solvent
Dispensing Solvents: Up to 7 types, including n-octanol, dodecane, toluene
Co-Solvents: Up to 10 types, including methanol, acetonitrile, DMSO, and MDM. Automated, six-way valve can add 6 types.
Dispenser: Precision dispenser, 0.5 ml syringe, multi-capillary bundle, minimum volume 0.024 L
Moving Probe: Fully automated X-Y axis titration module
Cleaning: Static and dynamic cleaning stations
Auto-Sampler: Optional, 192 sample positions, automatic mechanical gripper
Purge Gas: 2 internal flow meters, nitrogen or argon
Experimental Volume: 1.0-3.5 mLs
Environmental Requirements: Indoor use, altitude ≤2000m, temperature 5℃-40℃, maximum relative humidity 80%@31℃
Power Supply Voltage Fluctuation: +/-10%, overvoltage Cat, pollution degree 2.