Product summary

Rebus Esper™ Is a fully integrated, automated instrument that brings together advances in imaging, hydrology, chemistry, and bioinformatics to provide spatial context and subcellular resolution of high-throughput, quantitative, single-molecule, single-cell data, and can target up to 30 customized genes at a time

Data from the Rebus system will be interoperable and can be used to compare methods such as cross-RNA sequencing, single-cell RNA-seq, ChIP-seq, ATAC-seq, and proteomics.

Advantage

Designed to provide automated analysis of various biomolecules at subcellular resolution directly within and across large tissue sections. The platform combines a fully automated microfluidic setup with a proprietary synthetic aperture optics (SAO) system at its core for continuously labeled expression transcripts and sensitive, scalable high-resolution imaging. The first applications developed by the platform focus on spatial transcriptomics, where single-molecule fluorescence in situ hybridization (smFISH) chemical detection allows high levels of multiplexing, without the need for barcodes or enzyme amplification, to resolve and map individual RNA molecules at their original locations. It currently allows analysis of up to 30 custom genes

Application

Spatial transcriptomics

Single molecule Fluorescence in situ hybridization (smFISH) chemical detection

RNA sequencing

single cell RNA-SEQ

ChIP-seq

ATAC-seq

Detailed Specifications