Product Advantages:

FULLY AUTOMATED: Our tools completely automate the fabrication process, allowing you to make and quality control nanopores with the push of a button.

AFFORDABLE AND ACCESSIBLE: Our instruments deliver functional nanopores for a fraction of the cost and effort of other methods.

ACCURATE AND PRECISE: Our optimized protocols deliver nanopores with sizes in the 1-30 nm range automatically, with sub-nm precision.

Application：

Northern Nanopore's mission to accelerate solid-state nanopore research began with our patented controlled breakdown method for fabricating solid-state nanopores, and has expanded into AI/ML data analytics, cost-reducing experimental consumables, and focused in-house R&D toward single-molecule proteomics.

Detailed Specifications

Specification

The Spark-E2 is a solid-state nanopore fabrication unit that uses the cutting-edge Controlled Breakdown method. By allowing for the fabrication of two nanopores, this compact tool provides users with the pores they need to perform a wide range of single-molecule experiments in a rapid, simple, and cost-effective manner.

Number of Channels: 2

Current Range:± 1 µA (Fabrication Mode),±50 nA (Noise Measurement Mode)

Voltage Range ±17 V

Power Supply 110 V or 220 V AC

Size 172 mm x 80 mm x 48 mm

Weight ~2.5 kg

Optimal Conditions 10-30 nm SiNx

These Sensing Cages interface NNi flow cells with most commercial current amplifiers while protecting sensing experiments from electrical noise. Adaptations are available on demand for amplifiers not already supported.

Compatible with the following commercial amplifier head-stages:

Molecular Devices, Axopatch 200B; Multiclamp 700B

Elements srl, eONE, Nanopore Reader, 10 MHz Amplifier

Chimera Instruments, see page 6 for adapter for VC100

HEKA, HEKA EPC

Warner, BC535

Customizations available for other amplifiers on request.

Flow Cells: These reusable flow cells are specifically designed to be used with the Spark-E2 nanopore fabrication unit and with NNi sensing cages. Flow-through design and small internal volumes facilitates buffer exchange and minimizes sample waste.

Volume (clipping part)： 25 µL

Volume (socket part) ：40 µL

Assembled Size： 27 mm x 16 mm x 23 mm

Fastener： Screw

Powerful analysis tools simplify and accelerate postprocessing of nanopore data. NNi’s suite of tools is designed specifically with nanopore research in mind, with an ever-growing list of features driven by feedback from our users.

Operating System: Windows.

Supported Input Data Formats: bin (NNi software), .abf (ABF2, and some ABF1) .log (Chimera VC100), .dat (Elements eOne), and arbitrary binary formats

Output Format: csv

Accessories:

Disposable Ag/AgCl electrodes provide low-noise current sensing with no maintenance requirements.

Caps compatible with NNi electrodes form an air-tight seal that prevents evaporation for months.

Precisely cut gaskets seal the membrane in the flow cell and direct the flow of sample solution.

This tough PTFE jig holds up to 19 chips and fits in a 100mL beaker for easy cleaning with Piranha or other solvents.